Quantifying the Frequency of Tumor-propagating Cells Using Limiting Dilution Cell Transplantation in Syngeneic Zebrafish
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Quantifying the Frequency of Tumor-propagating Cells Using Limiting Dilution Cell Transplantation in Syngeneic Zebrafish
Self-renewing cancer cells are the only cell types within a tumor that have an unlimited ability to promote tumor growth, and are thus known as tumor-propagating cells, or tumor-initiating cells. It is thought that targeting these self-renewing cells for destruction will block tumor progression and stop relapse, greatly improving patient prognosis. The most common way to determine the frequency...
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Radioresistance mechanisms of mammary tumor initiating cells: unreliable biological conclusions based on limiting dilution assays.
The recent study of tumor initiating cells (TICs) in a p53 null mammary tumor model by Zhang et al. (1) emphasizes that the selective activation of the Akt and canonical Wnt/β-catenin signaling pathways within the tumorigenic TICs plays a critical role in radiation resistance of TICs. Limiting dilution experiments are used to estimate and to compare TIC frequencies in their protocols. As such, ...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2011
ISSN: 1940-087X
DOI: 10.3791/2790